Journal: Journal of Pharmacy & Bioallied Sciences
Article Title: Correlation Between Histological Grading and Ploidy Status in Oral Leukoplakia, Oral Submucous Fibrosis, and Oral Squamous Cell Carcinoma: A Flow Cytometric Analysis
doi: 10.4103/jpbs.JPBS_33_20
Figure Lengend Snippet: High-resolution flow cytometry
Article Snippet: Later the wrapped sections were digested in subtilisin Carlsberg solution at a temperature of 37°C in a centrifuge tube for 120 min and then agitated for 20 min. After this the Carlsberg solution was subjected to filtration method with the help of 50 µm mesh of nylon, and DAPI (4′,6-diamidino-2-phenylindole) staining solution was summated with the help of PAS II Flow Cytometry tool (Partec, Munster, Germany); Equipped with a 100 W mercury lamp [ ] and FCM procedure was carried out.
Techniques: Flow Cytometry